Fluorescence-Quenching Peptide Substrates |
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| Fluorescence-quenched
peptide substrates have been used to quantitate MMP activity. Fluoroesence resonance
energy transfer (FRET) occurs when the excitation energy is transferred from an excited
fluorescent donor to a quenching acceptor in a distance-dependent manner. Cleavage of a
scissile peptide bond within a fluorescence quenched substrate leads to the separation of
the intramolecular donor-acceptor pair, thus allowing the increase of the fluorescence.
The fluorescence increase is then proportional to the amount of peptide hydrolyzed. Popular FRET donor-acceptor pairs Abz-Leu......Dap(Dnp).... Abz-Gly-......EEDnp Dnp-Pro-....Lys(NMa).... MOCAc-Pro-......Lys(Dnp).... Abz =o -amino-benzoic acid; EEDnp = N-(2,4-dinitrophenyl)ethylenediamine; Dnp = 2,4-dinitrophenyl; NMa = Ne-Methylanthranoyl; MOCAc = (7-methoxycoumarin-4-yl) acetyl; |
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CODE |
PRODUCT |
QTY |
USD |
| SFQ-3817-PI | Abz-Gly-Phe-Ser-Pro-Tyr(NO2)-OH Fluorescence-Quenching Substrate for ACE2 (TBC5180) |
1 mg |
45 |
| SFQ-3819-PI | Abz-Ser-Pro-Tyr(NO2)-OH Fluorescence-Quenching Substrate for ACE2 (TBC5182) |
1 mg |
45 |
| SDP-3818-PI | Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser- Arg-Dap(Dnp)-NH2 2-Aminobenzoyl-L-Leucyl-L-Alanyl-L-Glutaminyl-L-Alanyl- L-Valyl-L-Arginyl-L-Seryl-L-Seryl-L-Seryl-L-Arginyl- [Ne-(2,4-Dinitrophenyl)-L-2,3-Diaminopropionyl] Amide Fluorescence-Quenching Substrate for ADAM17/TNF-a Converting Enzyme |
1 mg |
75 |
| SDP-3815-PI | Dnp-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(NMa)-NH2 2,4-Dinitrophenyl-L-Prolyl-L-Cyclohexylalanyl-Glycyl-S-Methyl- L-Cysteinyl-L-Histidyl-L-Alanyl-Ne-Methylanthranoyl-L-Lysine Amide Substrate for Collagenase |
2.5
mg |
165 |
SDP-3820-PI |
Dnp-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 2,4-Dinitrophenyl-L-Prolyl-L-Leucyl-Glycyl-L-Leucyl- L-Tryptophyl-L-Alanyl-D-Arginine Amide Substrate for Collagenase/Gelatinase |
2.5
mg |
95 |
SMO-3216-v |
MOCAc-Ala-Pro-Ala-Lys-Phe-Phe-Arg-Leu-Lys(Dnp)-NH2 (7-Methoxycoumarin-4-yl) acetyl-L-Alanyl-L-Prolyl-L-Alanyl- L-Lysyl-L-Phenylalanyl-L-Phenylalanyl-Arginyl-L-Leucyl- Ne-(2,4-Dinitrophenyl)-L-Lysine Amide Substrate for Proteinase A / Pepsin |
1 mg vial | 120 |
SMO-3167-v |
MOCAc-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2 [NFF-2] (7-Methoxycoumarin-4-yl) acetyl-L-Arginyl-L-Prolyl-L-Lysyl-L-Prolyl- L-Tyrosyl-L-Alanyl-L-Norvalyl- L-Tryptophyl-L-Methionyl-Ne-(2,4-Dinitrophenyl)-L-Lysine Amide Fluorescence-Quenching Substrate for Matrix Metalloproteinases |
1 mg vial |
107 |
SMO-3168-v |
MOCAc-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2
[NFF-3] (7-Methoxycoumarin-4-yl)acetyl-L-Arginyl-L-Prolyl-L-Lysyl-L- Prolyl-L-Valyl-L-Glutamyl-L-Norvalyl-L-Tryptophyl-L-Arginyl- Ne-(2,4-Dinitrophenyl)-L-Lysine Amide Fluorescence-Quenching Substrate for Matrix Metalloproteinase-3 (Stromelysin 1) |
1 mg vial |
107 |
MOC-3184-v |
MOCAc-Asp-Glu-Val-Asp-Ala-Pro-Lys(Dnp)-NH2 (7-Methoxycoumarin-4-yl)acetyl-L-Aspartyl-L-Glutamyl-L- Valyl-L-Aspartyl-L-Alanyl-L-Prolyl-Ne-(2,4-Dinitrophenyl)- L-Lysine Amide Fluorescence-Quenching Substrate for Caspase-3 |
1 mg vial |
163 |
SMO-3200-v |
MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu- Lys(Dnp)-D-Arg-NH2 (7-Methoxycoumarin-4-yl)acetyl-Glycyl-L-Lysyl-L-Prolyl- L-Isoleucyl-L-Leucyl-L-Phenylalanyl-L-Phenylalanyl-L- Arginyl-L-Leucyl-Ne-(2,4-Dinitrophenyl-L-Lysyl)-D-Arginine Amide Fluoresence-Quenching Substrate for Cathepsin D and E |
1 mg vial |
173 |
| MOCAc-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 Substrate for MMPs, Cathepsin D and E, ADAM10 and ADAM17/TACE Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (FS-6 from Neumann et al.) U. Neumann , H. Kubota, K. Frei, V. Ganu, and D. Leppert, Anal. Biochem., 328, 166 (2004). |
1 mg |
80 |
|
MOC-3164-v |
MOCAc-Pro-Leu-Gly (7-Methoxycoumarin-4-yl)acetyl-L-Prolyl-L-Leucyl-Glycine Reference Compound for Code 3163-v |
0.1 mg vial |
22 |
MOC-3163-v |
MOCAc-Pro-Leu-Gly-Leu-A2pr(Dnp)-Ala-Arg-NH2 (7-Methoxycoumarin-4-yl)acetyl-L-Prolyl-L-Leucyl-Glycyl- L-Leucyl-[Ne-(2,4-Dinitrophenyl)-L-2,3-Diaminopropionyl]- L-Alanyl-L-Arginine Amide Fluorescence-Quenching Substrate for Matrix Metalloproteinases |
1 mg vial |
65 |
SMO-3212-v |
MOCAc-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe- Arg-Lys(Dnp)-Arg-Arg-NH2 (7-Methoxycoumarin-4-yl)acetyl-L-Seryl-L-Glutamyl-L-Valyl- L-Asparaginyl-L-Leucyl-L-Aspartyl-L-Alanyl-L-Glutamyl-L- Phenylalanyl-L-Arginyl-Ne-(2,4-Dinitrophenyl)-L-Lysyl-L- Arginyl-L-Arginine Amide Fluoresence-Quenching Substrate Flanking b -Cleavage Site of Swedish-Type Amyloid Precursor Protein |
1 mg vial |
163 |
MOC-3183-v |
MOCAc-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-NH2 (7-Methoxycoumarin-4-yl)acetyl-L-Tyrosyl-L-Valyl-Alanyl -L-Aspartyl-L-Alanyl-L-Prolyl-Ne-(2,4-Dinitrophenyl)- L-Lysine Amide Fluorescence-Quenching Substrate for Caspase-1 |
1 mg vial | 163 |
Please contact Peptides International for ordering information. |
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